Hearing the familiar cry of the new beekeeper at the moment "I can't find the queen!"

What are your sure fire methods for locating that queen you need to find but haven't spotted the last 2 times you went through the frames and started to look for her?

I'll start off easy, before I do anything else I stop looking closely at the bees and look for her movement. She moves differently to the workers and her back legs tend to catch my attention as she scurries, it is nearly always the movement of her legs that I spot first.

I also tend to concentrate my search, if I haven't done too much shaking of bees, to frames containing eggs.