After trying grafting of larvae I much prefer the Nicot Cupkit sytem for bee breeding. This is my second year using this system and it works really well. This year the mother queen layed the eggs more easily than last year. I think this is due to the Cupkit being a bit older and not as 'clean'. I checked the cups yesterday and could still see the eggs had not hatched. They were however a little bigger than on day 1 when they were laid.
I left them for another 24hrs and today found they had hatched and were floating in a tiny amount of clear nectar that the house bees had placed in the cups. I transferred 10cups to a cell cup frame and placed them in a queenless colony to draw out the queen cells. Will report back on how many are taken up. I like this system as you can easily see the eggs and you can age the larvae and calculate when the Queens will hatch. The best bit is you can easily get 1 day old larvae without any mechanical handling of the larvae.