ABSTRACT Traditional morphometrics, allozymes, and mitochondrial data have supported a close relationship between the M branch subspecies
A. m. iberiensis and the North African subspecies (A branch). However, studies using nuclear DNA markers have revealed a clear distinction
between the latter and the two European M branch subspecies. In help resolve this paradox, we analyzed 663 colonies from six
European and African subspecies. A geometric morphometrics approach was applied to the analysis of wing shape, and the results
were compared with data of six microsatellite loci. Both data sets were found to be highly consistent and corroborated a marked
divergence of West European subspecies from North African ones. This supports the hypothesis that the presence of the African
lineage mitotype in Iberian honey bee populations is likely the consequence of secondary introductions, with a minimal African
influence within the current Iberian genetic background. Wing geometric morphometrics appears more appropriate than mitochondrial
DNA analysis or traditional morphometrics in the screening and identification of the Africanization process.
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